Iris de Nie

77 SUCCESSFUL RESTORATION OF SPERMATOGENESIS FOLLOWING GENDER-AFFIRMING HORMONE THERAPY 5 Patient 5 Patient 6 Patient 7 Patient 8 Patient 9 cis-gender female partner got pregnant 20 months after cessation of hormone treatment after failing to identify any mature sperm on repeated semen specimens, further physical examination and investigations were performed. Examination revealed a normal penis, right testicle volume of 2 ml, left testicle volume of 3 ml and normal consistency on both sides without any abnormalities of the epididymis or vas deferens. Scrotal ultrasound revealed: right testicle volume of 2.04 ml, left testicle volume 3.66 ml, normal testicular structure and vascularisation, normal epididymis. Karyotype was 46XY with no Y chromosome abnormalities seen. testicular sperm extraction was subsquently performed. Complete spermatogenesis was sporadically encountered in both testicles, and mature spermatozoa were harvested 17 months after stopping hormones.