Sanne de Bruin

136 Chapter 5 hours-7 days and 24-72 hours, respectively. Data on PPP activity and glutathionemetab- olism after transfusion are still lacking. Investigating these compounds is a prerequisite to improve current storage conditions. Techniques, such as metabolomics, to determine metabolite levels after storage have greatly increased our knowledge on the behaviour of different metabolites during storage. Ideally, metabolite levels after transfusion in fresh and stored RCCs should be compared using different additive solutions, which has now become realistic through the emergence of metabolomics approaches that require much less cells than previous methods. Data obtained in this sort of analyses could lead to new insights to further improve storage techniques and it could confirm or refute the hypothesis that metabolic changes during storage are clinically relevant.