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152 Chapter 6 Results Clinical effects Twenty subjects were transfused with 25 ml 35 days stored autologous RBCs, either stored in SAGM or PAGGGM, divided in groups of 10 volunteers each. As a reference, 25 ml 2 days stored autologous RBCs, all in SAGM, were simultaneously transfused. In 5 subjects only the 35 days stored RBCs were administered due to logistic issues concerning the 2-day stored RBCs. Four of these subjects were allocated to the SAGM group and one subject who received only RBC concentrate was allocated to the PAGGGM group. No adverse events were reported. After transfusion of the RBCs, no effects on bilirubin, haptoglobin, lactate dehydrogenase (LDH) and aspartate aminotransferase (ASAT) were observed in either group (Figure S2). In addition, transfusion of SAGM-RBCs and PAGGGM-RBCs did not affect body temperature, blood pressure, respiratory rate and heart rate during the study day (data not shown). Storage in PAGGGM does not result in higher PTR The PTR of 35 days stored PAGGGM-RBCs was compared with the PTR of 35 days stored SAGM-RBCs. The PTR was significantly lower for the PAGGGM-RBCs over 90 days (Figure 1, p=0.002). However, in the post-hoc analysis of each separate timepoint, no significant differences were observed (supplement table S1). The 24 hours PTR of 35 days stored PAGGGM-RBCs was 68% [59-87] and 81% [70-93] in SAGM stored RBCs (p=0.16). 35 days of storage results in a decreased PTR As anticipated, the duration of storage had a significant effect on the PTR of 35 days stored RBCs in SAGM (p<0.05, Figure 1B.). Post hoc analysis showed that the PTR was significantly higher in 2 days stored RBCs at 2 days (96% [90-99] versus 72% [66-89], p<0.05) and seven days (96% [90-99] versus 72% [66-89], p<0.05) after transfusion compared to the 35 days stored RBCs. The 24 hours PTR of 2 days stored SAGM-RBCs was 97% [97-105] and 81% [70-93] in 35 days SAGM-RBCs (p=0.056). A similar effect was observed in the PAGGGM group. Here we compared 2 days stored RBCs in SAGMwith 35 days stored RBCs in PAGGGM. The 35 days stored RBCs had a sig- nificant lower PTR over time (p<0.01). In this post-hoc analysis, the PTRwas significantly lower at 8 hours, 2 days, 30 days and 90 days after transfusion (supplemental table S2).