Sanne de Bruin

181 Biotinylation of platelets for transfusion purposes: a novel method to label platelets in a closed system Figure 1. Biotinylation of platelets and biotinylation procedure. A. Platelets (top left), are incubated with sulfo-NHS-biotin (top middle), bioPLTs are counterstained with fluorescently labeled streptavi- din (top right). Unlabeled platelets show a characteristic FACs histogram, the fluorescently labeled biotin-streptavidin causes the peak of the histogram to shift horizontally. B. A proportion of the PC was washed twice in PAS-E. Prior to each washing step, the PC was acidified to 10% ACD-A. Platelets were incubated with 5 mg/L biotin, diluted in PBS-PAS-E (1:9), for 30 minutes. BioPLTs were washed and resuspended in PAS-E. 7