Sanne de Bruin

187 Biotinylation of platelets for transfusion purposes: a novel method to label platelets in a closed system Figure 3. Biotinylation in various conditions. A. Biotinylation performed with biotin stored in PBS for 42 days at -30. There was no difference between freshly dissolved biotin and a stored biotin solution (mean of 97.9 and 97.1% respectively, n=3). B. Adding ACD-A simultaneoulsy with the biotin, led to a decrease of the amount of bioPLTs (mean of 88.5% versus 97.10%, n=3). C. Irradiation with 25 Grey did not influence the amount of bioPLTs (mean of 98.1%) . 7