Sanne de Bruin

198 Chapter 7 Appendix 2. Supplemental figures Figure S1. A) Procedural steps to obtain biotinylated platelet, sham platelets and the control sample. B) Validation experiment in which platelets were labeled at day 1 or day 7 of storage. Platelets labeled on day 1 of storage were also stored for 3 days separately to assess the effect of storage on the quality of the platelets. However this method of storage led to unacceptable high platelet activation. Therefor another method was designed: C) After labeling, the bioPLT were returned in its original bag. Here, the various parameters tested in biotinylated platelets fulfilled the quality criteria.