111 TSPO in neurodegeneration Table S6. Antibodies for immunohistochemistry and imaging mass cytometry Antigen Species (isotype) Clonality Dilution Antigen Retrieval Product Number Supplier TSPO goat pAb 1:750 Citrate NB100-41398 Novus Biologicals TSPO rabbit mAb 1:750 Citrate AB109497 Abcam IBA1 rabbit pAb 1:10000 Tris-EDTA 019-19741 Wako IBA1 goat pAb 1:1000 Tris-EDTA AB48004 Abcam IBA1 guinea pig pAb 1:100 Citrate 234004 Synaptic Systems GFAP chicken pAb 1:500 Citrate AB5541 Millipore HLA-DR mouse (IgG2B) mAb 1:750 Citrate 14-9956-82 Invitrogen Aβ IC16 mouse (IgG2A) mAb 1:400 Citrate N/A in-housea P-Tau AT8 mouse (IgG1) mAb 1:400 Citrate AB_223647 Invitrogen PLP mouse (IgG2A) mAb 1:200 Citrate MCA839G Bio-Rad IMC Ln-Isotope CD68 159Tb 1:800 EDTA 3159035D Fluidigm GFAP 162Dy 1:600 EDTA Ab218309 Abcam HLA-DR 174Yb 1:400 EDTA 3174025D Fluidigm IBA1 169Tm 1:3000 EDTA 019-197471 Wako TSPO 149Sm 1:400 EDTA Ab213654 Abcam aWith permission from Carsten Korth, Heinrich Heine University, Düsseldorf, Germany. Abbreviations: CD – cluster of differentiation; GFAP – glial fibrillary acidic protein; IBA1 – ionized calcium-binding adaptor molecule 1; mAb – monoclonal antibody; pAb – polyclonal antibody; P-Tau – phosphorylated Tau (Ser202, Thr205).
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