Lower plasma magnesium, measured by nuclear magnetic resonance spectroscopy, is associated with increased risk of developing type 2 diabetes mellitus in women: Results from a Dutch prospective cohort study 27 2 Figure 1. Flowchart of the PREVEND study participants included or excluded for the purposes of this study. NMR-based ionized Mg assay As is customary for collecting NMR spectra for the NMR LipoProfile test, EDTA plasma samples were diluted 1:1 with phosphate buffer (pH 7.4) containing 5 mmol/L EDTA. The extra EDTA in the buffer ensured complete chelation of free ionized Mg present in the plasma specimens, as well as any circulating Mg that may not be tightly bound to proteins, citrates or phosphates. Proton NMR spectra were collected on 400 MHz Vantera Clinical Analyzers at 47°C as described previously 16,21. The NMR acquisition time was 48 seconds, with a total sample to sample turnaround time of 90 seconds.
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