70 Chapter 3 Supplementary table S2: MET antibody validation cell line panel culture conditions. Cell line T (⁰C) CO2 (%) Medium Supplements HeLa 37 5 DMEM/F12 (Life TechnologiesTM) 10% Fetal Calf Serum (Life TechnologiesTM) and 1% Penicillin/Streptomycin (Life TechnologiesTM) SK-BR-3 37 5 RPMI 1640 (Life TechnologiesTM) 10% Fetal Calf Serum (Life TechnologiesTM) and 1% Penicillin/Streptomycin (Life TechnologiesTM) HT-29 37 5 RPMI 1640 (Life TechnologiesTM) 5% Fetal Calf Serum (Life TechnologiesTM) and 1% Penicillin/Streptomycin (Life TechnologiesTM) LNCaP 37 5 DMEM/F12 (Life TechnologiesTM) 10% Fetal Calf Serum (Life TechnologiesTM) and 1% Penicillin/Streptomycin (Life TechnologiesTM) PC3 37 5 DMEM/F12 (Life TechnologiesTM) 10% Fetal Calf Serum (Life TechnologiesTM) and 1% Penicillin/Streptomycin (Life TechnologiesTM) DU145#Sh167 37 5 RPMI 1640 (Life TechnologiesTM) 5% Fetal Calf Serum (Life TechnologiesTM), 1% Penicillin/Streptomycin (Life TechnologiesTM) and 0.5 mg/mL Puromycin (Sigma-Aldrich®) DU145 37 5 RPMI 1640 (Life TechnologiesTM) 5% Fetal Calf Serum (Life TechnologiesTM) and 1% Penicillin/Streptomycin (Life TechnologiesTM) Supplementary table S3: MET antibody titers used during different applications. Clone Western blot Immunocytochemistry IHC FFPE cell lines IHC FFPE tissues D1C2 1:1000 1:1000 1:400 1:100 SP44 1:1000 1:1000 1:800 1:200 CVD13 1:1000 1:1000 1:800 1:200 C-12 1:1000 1:1000 1:800 1:200 C-28 1:500 1:500 1:400 1:100 Abbreviation: IHC, immunohistochemistry.
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