showing the limits of agreement for concordant corresponding tissue core scores of SW & MDH. The limits of agreement are the upper and lower limits of the interval d ± 1.96sd where d is the mean of the differences between corresponding observations of SW and MDH and sd its standard deviation. Before reassessment there were 82 – depicted between brackets – discordant scores (red circles) (F). After reassessment this number is reduced to 3 (G). Abbreviations: CS, Concordant Score; DS, Discordant Score. Supplementary figure S2: Immunoreactivities of the five tested C-terminal MET antibodies under native conditions in respect to MET mRNA expression levels across the antibody validation cell line panel. A. representative immunoreactivities of all antibodies with all cell lines and the corresponding negative controls (NCs). B. membranous (M), cytoplasmic (C), and nuclear (N) immunocytochemical reactivity. C. qRT-PCR results showing average MET fluorescence standardized to average HPRT1 fluorescence. D. Legend for observed mRNA expression levels and immunocytochemical reactivities. It should be noted that pictures used to present the immunoreactivities obtained with D1C2 and CVD13 are the same as those presented in Figure 1C.
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