90 Chapter 4 Table 1: Definition of staining intensities (SIs) and scores used to evaluate D1C2 and A2H2-3 immunoreactivity across cell lines, tissues, and tissue microarrays. Evaluation of MET ectodomain shedding across formalin-fixed paraffin-embedded (FFPE) tissues and tissue microarrays. Evaluation of MET ectodomain shedding across tissue microarrays. Definition of staining intensities and scores used to evaluate D1C2 and A2H2-3 immunoreactivity across FFPE cell lines and tissues Cellular localization SI Description SI Score Description score Nucleus and cytoplasm 0 No staining 0 <10% of cancer cells have SI 1, 2, or 3 >90% of cancer cells have SI 0 1 Weak staining 1 <10% of cancer cells have SI 2 or 3 ≥10% of cancer cells have SI 1, which is maximal observed SI 2 Moderate staining 2 <10% of cancer cells have SI 3 ≥10% of cancer cells have SI 2, which is the maximal observed SI 3 Strong staining 3 ≥10% of cancer cells have SI 3, which is the maximal observed SI Membrane 0 No staining 0 <10% of cancer cells have SI 1, 2, or 3 >90% of cancer cells have SI 0 1 Weak complete or incomplete membranous staining 1 <10% of cancer cells have SI 2 or 3 ≥10% of cancer cells have SI 1, which is maximal observed SI 2 Moderate complete membranous staining 2 <10% of cancer cells have SI 3 ≥10% of cancer cells have SI 2, which is the maximal observed SI 3 Strong complete membranous staining 3 ≥10% of cancer cells have SI 3, which is the maximal observed SI Evaluation of MET ectodomain shedding across FFPE tissues SI D1C2 and A2H2-3 Score D1C2 and A2H2-3 Description ectodomain shedding Score ectodomain shedding Description score 0 1 Negative % of cancer cells negative for A2H2-3, while positive for D1C2 Negative <10% of cancer cells display ectodomain shedding 2 3 Positive Positive ≥10% of cancer cells display ectodomain shedding
RkJQdWJsaXNoZXIy MTk4NDMw