92 Chapter 4 Fig. 1: Images of membranous A2H2-3 immunoreactivities observed for tissues (×20 objective). The percentage of cells subjective to ectodomain shedding across whole tissue sections was assessed using the differences between C- and N-terminal MET immunoreactivity observed using parallel sections (Table 1). Scoring of D1C2 and A2H2-3 immunoreactivity, MET protein status, and ectodomain shedding across the tissue microarray Scoring of membranous D1C2 and A2H2-3 across the tissue microarray was performed as described in Table 1. Seemingly suboptimal fixed cores were omitted during scoring. Disagreement between the two observers was assessed using a Bland and Altman diagram and reevaluation was performed simultaneously until agreement was reached. To evaluate the consistency of A2H2-3 immunoreactivity within the cancer center and periphery, the intraclass correlation coefficient was calculated for both regions. To evaluate the difference between D1C2 as well as A2H2-3 immunoreactivity scored in the center and periphery, a paired t-test was performed. Calculations were performed with SPSS Statistics (version 24; IBM; Armonk, NY, USA). Unless otherwise mentioned, statistical significance was set at P value < 0.05. Bubble plots used to illustrate N-terminal MET immunoreactivity across cancer surfaces were generated with Microsoft® Excel (version 2010; Microsoft®; Redmond, WA, USA).
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