99 MET ECD shedding and poor DFS Fig. 5: Occurrence of MET ectodomain shedding and regulated intramembrane proteolysis in corresponding oral squamous cell carcinoma fresh frozen and formalin-fixed paraffin-embedded tissues. The abbreviations RIPping, WB, and IHC used in this figure stand for regulated intramembrane proteolysis, western blot, and immunohistochemistry. a Heatmap depicting observed immunoreactivities for C-terminal MET protein products and fragments (Supplementary Tables 4, 5) under reducing conditions observed with D1C2 and a ribbon depicting shedding and/or regulated intramembrane proteolysis status per tissue specimen based on D1C2 immunoreactivity under reducing conditions. (1) Negative for p145β and C-terminal fragments. (2) Positive for p145β and negative for C-terminal fragments. (3) Positive for p145β and C-terminal fragments that are the result of ectodomain shedding. (4) Positive for p145β and C-terminal fragments that are the result of regulated intramembrane proteolysis. (5) Positive for p145β and C-terminal fragments that are the result of ectodomain shedding and regulated intramembrane proteolysis. b Shedding status under reducing conditions per cancer specimen based on the results depicted in a and shedding status based on difference in immunoreactivity positivity between D1C2 and A2H2-3 (formalin-fixed paraffin-embedded). Cancers were scored positive for ectodomain shedding if at least 10% of the cancer cells are subjective to ectodomain shedding. c Legend for observed D1C2 immunoreactivities under reducing conditions and occurrence of MET ectodomain shedding under reducing and formalin-fixed paraffin-embedded conditions. 4
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