Chapter 4 108 SUPPLEMENTARY DATA Supplementary Figure S1 - Calibration of MESF detection of IFCM based on standardized fluorescent beads. (A ~ C) IFCM measured 5 populations of beads per kit with standardized MESF-Alexa488 (A), MESF-PE (B), or MESF-APC (C). (D ~ F) The regression analysis between log MESF and log MFI for Alexa488 (D), PE (E), or APC (F). Regression formulas were calculated based on non-blank populations (P1-4), and the R2 denoted the regression coefficient. Marks: ***p < 0.001; **p < 0.01. Abbreviations: MESF, molecules of equivalent soluble fluorochrome; MFI, median fluorescent intensity; IFCM, imaging flow cytometry; PB: blank population.
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