Isolation-Free Measurement of Single Urinary Extracellular Vesicles by Imaging Flow Cytometry 4 113 Supplementary Figure S7 - Inter- and intra-assay reproducibility to detect CD63+ uEV by IFCM. (A - C) Individually repeated IFCM experiments measured the concentration, MESF-Alexa488, and MESF-APC of CD63+ EV from the positive control (COLO205 cell line supernatant) on 14 separate days. The CV represented the reproducibility of IFCM. (D - F) One IFCM experiment repeatedly measured the concentration, MESF-Alexa488, and MESF-APC of CD63+ EV from the positive control (n=1) and healthy urine sample (n =3) at each hour. The range of CV was shown (n =4). Abbreviations: CV, coefficient of variation; MESF, molecular equivalent soluble fluorochrome; MFI, median fluorescence intensity; PC, positive control.
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