Chapter 4 114 Supplementary Figure S8 - The influence of dilution on urinary pH and its effect on uEV detection by IFCM. (A) The effects of dilution on the total numbers of CD63+ uEV by IFCM. Marks: ***p < 0.001; **p < 0.01. (B) The total numbers of uEV were calculated by correcting the concentrations with dilution times. Marks: ns, no significance. Abbreviations: A-F, autofluorescence; IFCM, imaging flow cytometry. Supplementary Figure S9 - Comparison between IFCM and TR-FIA. (A) Correlation (linear regression) analysis between CD63+ uEV concentrations by IFCM and CD63 expression levels in healthy urine samples (n = 5). (B and C) Correlation (linear regression) analysis between the MESF of CD63+ uEV by IFCM and CD63 expression levels in healthy urine samples (n = 5). R2 denoted the coefficient of regression. Marks: *p < 0.05; ns, no significance. Abbreviations: Eu, europium; IFCM, imaging flow cytometry; TR-FIA, time-resolved fluoroimmunoassay.
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