Isolation-Free Measurement of Single Urinary Extracellular Vesicles by Imaging Flow Cytometry 4 115 Clinical parameters Healthy controls (n = 5) Kidney transplant recipient with acute kidney injury in 2 weeks post-transplantation (n = 5) Gender (male/female) 4/1 4/1 Pathology presented in biopsy - Rejection (n = 2); Acute tubular necrosis (n = 3) Age (year) 27.80 ± 1.30 63.40 ± 7.73 Urine pH 5.68 ± 0.98 6.20 ± 0.45 Urinary total protein concentration (g/L) 0.07 ± 0.04 0.65 ± 0.30 Urinary creatinine concentration (mmol/L) 16.46 ± 7.76 8.80 ± 4.77 Supplementary Table S1 - Clinical parameters of enrolled subjects. Built-in or self-made Feature Mask Built-in Intensity_MC_Ch02 MC Built-in Intensity_MC_Ch03 MC Built-in Intensity_MC_Ch05 MC Built-in Intensity_MC_Ch06 MC Self-made Spot Count Ch02 Peak (M02, Ch02, Bright, 1) Self-made Spot Count Ch05 Peak (M05, Ch05, Bright, 1) Self-made Spot Distance Min (Ch02 & Ch05) Peak (M02, Ch02, Bright, 1) Or Peak (M05, Ch05, Bright, 1) Supplementary Table S2 - All used features with MCs in the IFCM analysis.
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