Chapter 4 116 Population Beads-Alexa488 Beads-PE Beads-APC Median MESF Log MESF Median MFI Log MFI Median MESF Log MESF Median MFI Log MFI Median MESF Log MESF Median MFI Log MFI Blank population (PB) NA NA 6558.17 3.817 NA NA 6227.22 3.794 NA NA 1022.26 3.010 Population 1 (P1) 11489 4.060 35776.51 4.554 394 2.595 11489 4.10493 65829 4.818 11489 3.800 Population 2 (P2) 63949 4.806 157752.88 5.198 3997 3.602 63949 4.84879 224599 5.351 63949 4.385 Population 3 (P3) 311396 5.493 633621.63 5.802 14988 4.176 311396 5.400839 1200523 6.079 311396 5.055 Population 4 (P4) 1636893 6.214 2183260.06 6.339 35476 4.550 1636893 5.763067 4324094 6.636 1636893 5.488 Supplementary Table S3 - Characteristics of MESF-standardized beads. Manufacturers provided the MESF values, and the MFI values were obtained in the detection by IFCM. The MFI values of non-blank populations (P1-4) were used to calculate the MESF. Abbreviations: MESF, molecules of equivalent soluble fluorochrome; MFI: median fluorescence intensity; NA: not available.
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