Extracellular Vesicles are Associated with Human Donor Kidney Characteristics 5 127 Figure 2 – EV release by discarded kidneys during NMP. A) Representative scatter plots of a stained sample (top row) and the corresponding sample after detergent treatment (bottom row) for each time point measured (T0 / T60 / T180 / T360, minutes). B-D) Concentrations of single-positive CFSE, single-positive Tetraspanin and double-positive CFSE and Tetraspanin fluorescent objects/mL respectively, before and after detergent treatment per time point measured. Red dots/black lines representing the means/median values of each time point, respectively. Statistical analysis (unpaired t-test, two sided, (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)) showed significant release of EV for all time points with respect to pre-NMP samples (C and D); Only the Tetraspanin single positive population (C) showed a significant increase from 60 to 180 minutes. No significant differences in release were observed between the other time points/populations.
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