Direct Detection of Circulating Donor-Derived Extracellular Vesicles in Kidney Transplant Recipients 6 153 These data suggest that our IFCM protocol is able to directly detect single CD9+ EVs in diluted plasma samples obtained from donors and KTRs before transplantation, despite the differences in molecular composition of the plasma between both groups. Figure 1 – Detection of CD9+ EVs in both (living) donor and kidney transplant recipient (KTR) samples obtained before transplantation. A) left to right: representative histogram plots of a PPP sample stained with anti-CD9, the same sample after detergent treatment (to discriminate between vesicular and non-vesicular events), and isotype staining. B) For each of the controls applied: quantification of total CD9+ EV concentrations as detected in PPP samples obtained from (living) donors, KTRs, and buffer only controls.
RkJQdWJsaXNoZXIy MTk4NDMw