Nanoparticle Release by Extended Criteria Donor Kidneys during Normothermic Machine Perfusion 2 25 Figure 1 - Renal nanoparticle release measured by NTA. A. Image of kidney derived nanoparticles during Nanoparticle Tracking Analysis (NTA) measurements. B. Size distribution vs. particle concentration of 1. perfusate after 0 minutes of perfusion and 2. perfusate after 120 minutes of perfusion. C. Ex vivo nanoparticle release by extended criteria donors (ECD) kidneys during Normothermic Machine Perfusion. Perfusate was obtained at 30 minutes intervals and measured with NTA. Accumulation of nanoparticles within the cumulative perfusion fluid was observed over time for all kidneys perfused, with highest excretion rate observed in the DBD kidney. REFERENCES 1. Brat A, Pol RA, Leuvenink HG. Novel preservation methods to increase the quality of older kidneys. Curr Opin Organ Transplant. 2015;20(4):438-443. 2. Hosgood SA, Saeb-Parsy K, Wilson C, Callaghan C, Collett D, Nicholson ML. Protocol of a randomised controlled, open-label trial of ex vivo normothermic perfusion versus static cold storage in donation after circulatory death renal transplantation. BMJ Open. 2017;7(1):e012237. 3. Morelli AE. Exosomes: From Cell Debris to Potential Biomarkers in Transplantation. Transplantation. 2017;101(10):2275-2276.
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