Chapter 3 44 Figure 5 – Examination of the accurate quantification of single EV detection by IFCM. Analysis of serial dilutions of 5 double-stained (CFDA-SE & anti-tetraspanin mixture) PPP samples showed a linear correlation between a) the obtained concentration and b) Equivalent number of Reference Fluorophores (ERF) of fluorescent detection channels Ch02 (CFSE) and Ch05 (APC) with dilution factor (4-fold).
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