TABLE OF CONTENTS Fantastic Vesicles and How to Find Them Chapter 1 General Introduction and Objectives 9 Chapter 2 Nanoparticle Release by Extended Criteria Donor Kidneys during Normothermic Machine Perfusion – Transplantation, 2019 23 Part I – Development: The Needle in the Haystack Chapter 3 An Imaging Flow Cytometry-Based Methodology for the Analysis of Single Extracellular Vesicles in Unprocessed Human Plasma – Communications Biology, 2022 29 Chapter 4 Isolation-free Measurement of Single Urinary Extracellular Vesicles by Imaging Flow Cytometry – Nanomedicine: Nanotechnology, Biology, and Medicine, 2023 83 Part II – Validation: Detection of EVs in Kidney Transplantation Chapter 5 Extracellular Vesicles Released During Normothermic Machine Perfusion are Associated with Human Donor Kidney Characteristics – Transplantation, 2022 121 Chapter 6 Direct Detection of Circulating Donor-Derived Extracellular Vesicles in Kidney Transplant Recipients – Scientific Reports, 2022 149 What have we learned? Chapter 7 Summaries 175 - English Summary 176 - Nederlandse Samenvatting 179 Chapter 8 General Discussion and Future Perspectives 183 Chapter 9 About the Author 199 - Curriculum Vitae 200 - List op Publications 201 - PhD Portfolio 202 - Acknowledgements 204
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