Chapter 3 60 Statistics and Reproducibility Statistical analysis was performed using R version 4.0.2 and RStudio (RStudio Team (2016). RStudio: Integrated Development for R. RStudio, Inc., Boston, MA; URL: http://www.rstudio.com/) version 1.1.463. All concentrations reported in this work were corrected for sample dilution (before acquisition – 380 µL total volume per test containing 30 µL sample = ~12.33-fold dilution factor) and are shown as the mean ± standard deviation unless specified otherwise. In all experiments conducted, PPP samples from the same 5 healthy individuals were used (n = 5 biologically independent samples). In the mouse vs human experiments, three independent experiments were conducted using the same mouse and human PPP samples (three replicates). ACKNOWLEDGMENTS The authors would like to thank Peter Rhein (Luminex) for his support and discussions throughout the project. AUTHOR CONTRIBUTIONS W.W.W. participated in the research design, execution of the research, data analysis, and article drafting and is the corresponding author. E.vd.P. participated in the calibration of scatter intensities through Mie theory. E.M. participated in data acquisition and provided IFCM expertise. M.J.H., C.C.B., and K.B. participated in the research design. A.M. participated in the research design, data acquisition and data analysis. All authors reviewed the manuscript and approved its final version. COMPETING INTERESTS The authors declare no competing interests. DATA AVAILABILITY All source data underlying the figures presented in this work are provided as ‘Supplementary Data 1-8’ (separate tabs for each figure). Any other relevant data are available from the corresponding author upon reasonable request.
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