An Imaging Flow Cytometry-Based Methodology for the Analysis of Single Extracellular Vesicles 3 65 SUPPLEMENTARY DATA Supplementary Figure 1 – Fluorescent calibration of BV421 a) The median fluorescent intensities (MFI) of each fluorescent peak of BV421 ERF calibration beads was measured with the same instrument/acquisition settings applied as used for EV acquisition. b) Calculation of the log of the MFI and ERF values (provided by the bead manufacturer). c) For both detection channels, the log of the MFI was plotted on the x-axis, and the log of the ERF values on the y-axis. A linear regression analysis was performed, respectively. d) Representative example of uncalibrated data (left) and corresponding ERF calibrated data (right).
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