Abbreviations Aztreonam broth Tryptic soy broth with aztreonam 75 mg/L CFU Colony forming units CPE Carbapenemase-producing Enterobacterales CP-PA Carbapenemase-producing Pseudomonas aeruginosa CR-AB Carbapenem-resistant Acinetobacter baumannii Erasmus MC Erasmus MC University Medical Center, Rotterdam, the Netherlands ESBL Extended-spectrum β-lactamase ESBL-E Extended-spectrum β-lactamase-producing Enterobacterales FCW Facility care worker HAI Healthcare-associated infections HRMO Highly resistant microorganisms ICU Intensive Care Unit IPC Infection prevention and control MALDI-TOF Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight mass spectrometry MIC Minimum inhibitory concentration MRSA Methicillin-resistant Staphylococcus aureus PBS Phosphate buffered saline RODAC Replicate Organism Direct Agar Contact-plates TSA Trypticase Soy Agar TSB Tryptic Soy Broth Vancomycin broth Tryptic soy broth with vancomycin 50 mg/L VIM-PA VIM-positive Pseudomonas aeruginosa VRE Vancomycin-resistant Enterococcus faecium WGS Whole genome sequencing 3 165 Environmental contamination with MDRO in single-occupancy rooms
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