Supplemental file 2: Detected AMR genes and heatmaps for ESBLproducing Escherichia coli and K pneumoniae Of the 16 patients included twice during the study period, two patients were positive for ESBL-E. Patient 128 was positive for an ESBL-producing E. coli at admission and discharge for both admissions. These E. coli strains were identical according to cgMLST, and no differences were observed in AMR genes. Patient 5 was positive for an ESBL-producing Citrobacter freundii at admission, but for an ESBL-producing K. pneumoniae at discharge. For the second hospitalization, the patient was positive for an ESBL-producing K. pneumoniae at admission and discharge. Details for isolates of E. coli (A) and K. pneumoniae (B), including patient number, sampling moment, conventional MLST results, cgMLST cluster types determined by SeqSphere+ software (Ridom, Munster, Germany) and presence of antibiotic resistance genes (search restricted to perfect and strict matches) as determined using the CARD web-interface (https://card.mcmaster.ca/). Results shown are focused on different types of betalactamases and aminoglycoside modifying enzymes. A grey background indicates patients with strains having different CARD results between admission and discharge despite having an isogenic chromosomal background. A yellow background indicates patients with strains of a different genetic background between admission and discharge. Pink indicates patients only testing positive at discharge. A black background indicates presence of the gene. Numbers indicate percentage identity to the CARD reference sequence. 2 47 Effect of single-occupancy rooms on ESBL-E acquisition
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