271 General discussion and future perspectives in situ slides we found an alveolar connection between adjacent secondary lobules in H&E staining supported by the elastin fibers in the elastin staining (see Figure 2 and 3) Figure 2. Upper left and right: hematoxylin & eosin-stained section from adenocarcinoma growing on alveolar walls, monolayer of tumor cells. T-junction of septa is present (blue lines and arrows). Note ‘crossing over’ of AIS at the septum (blue square). Lower left and right: same areas as above, showing elastin fibers in all preexisting alveolar septa (left) and in cytokeratin 7 staining the monolayer of tumor cells along the alveolar septa in a regular pattern and crossing over at a small ‘hole’ in the alveolar septum (blue square). The function of the alveolar connections in the septa is probably similar as that in the pores of Kohn and might be related to an overflow “pressure relief” function. 17
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