86 Chapter 6 Figure 1. Surface of cross-section from lobectomy resection specimen after the first (after overnight fixation) cut (slice A) and second cut (slice B); arrows indicate cutting direction. In rectangle tissue sampled with peripheral lung tissue above tumor (blocks 1 and 3) and below tumor (blocks 2 and 4). A super cial complete hematoxylin and eosin–stained slide from each tissue block was examined for the presence of loose tissue fragments at x10 or x20 magnification. Loose benign or malignant tissue, also called displaced fragment, was scored if the fragment was at least 0.5 mm (approximately one field of view of a x40 microscope objective) from the tumor (Fig. 2) or if it was on the pleura surface in the plane of the second cut.
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