227 The role of the MR 7 Figure 1. Hydrocortisone can induce expression of NR3C1 and NR3C2 via both the GR and MR. (A), Western blot analysis of DDK, NR3C1 and BIM in Reh cell lines that were transfected with either doxycycline-inducible DDK-tagged NR3C1 or NR3C2 constructs, after treatment with prednisone, dexamethasone or hydrocortisone, as indicated. (B), Transcriptional steroid response of Reh cell lines transfected with doxycycline-inducible NR3C1 or NR3C2 constructs. After doxycycline induction, cells were treated with 0.16µM dexamethasone or 0.032µM (RehNR3C1) or 0.0028µM (RehNR3C2) hydrocortisone. Expression of NR3C1 (upper panels) and NR3C2 (lower panels) was measured in both cell lines, as well as (C), expression of GR and MR target genes BIM, GILZ and FKBP5.
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