237 The role of the MR 7 SUPPLEMENTAL FIGURES Supplemental Figure 1. (A), Reh cells were transduced with a doxycycline-inducible NR3C1 or NR3C2 construct. The inducibility of the NR3C1- or NR3C2- constructs was measured through flow cytometry following intracellular DDK staining. Doxycycline exposure induced the expression of DDK-tagged NR3C1 (referred to as RehNR3C1) or NR3C2 (referred to as RehNR3C2) in Reh single cells that intrinsically lack NR3C1 and NR3C2 expression. (B), Western blot analysis of our secondary Reh cell lines (RehNR3C1-B and RehNR3C2-B) that were transfected with either doxycycline-inducible DDK-tagged NR3C1 or NR3C2 constructs, after treatment with prednisone, dexamethasone or hydrocortisone, as indicated.
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