197 An in vitro model of fibrosis using crosslinked native extracellular matrix-derived hydrogels to modulate biomechanics without changing composition Figure 5: Picrosirius red staining on LdECM and Ru-LdECM and fibre characteristics analysis on the collagen network. LdECM and Ru-LdECM hydrogels were stained using Picrosirius red staining and generated fluorescent images were analysed using TWOMBLI plugin in FIJI ImageJ. A) Representative images of Picrosirius red staining on LdECM and Ru-LdECM hydrogels, B) Average Fibre Length, C) Endpoints per 1000 μm total length, D) Branchpoints per 1000 μm total length, E) HGU, F) % of High Density Matrix (HDM), G) % fibre Alignment. Each dot represents the mean of measurements of 5 different randomized regions on the fluorescent images of Picrosirius red staining for each sample (n=5). Applied statistical test: paired t-test to compare the LdECM and Ru-LdECM hydrogels that were generated in the same experimental batch (as indicated by the connecting lines in the graph). LdECM: Lung-derived ECM Hydrogels, Ru-LdECM: Ruthenium-crosslinked Lung-derived ECM Hydrogels 8
RkJQdWJsaXNoZXIy MTk4NDMw