198 Chapter 8 Figure 6: Live/dead staining on MRC-5 fibroblasts seeded on LdECM and Ru-LdECM hydrogels on day 1 using Calcein AM (green) and propidium iodide (red). A) MRC-5 fibroblasts cultured on the LdECM hydrogels, B) MRC-5 fibroblasts cultures on Ru-LdECM hydrogels. Scale bar: 1000 μm. Results are representative for all experiments (n =5). LdECM: Lung-derived ECM Hydrogels, Ru-LdECM: Ruthenium-crosslinked Lung-derived ECM Hydrogels Table 6: TWOMBLI analysis for the curvature of fibres with different lengths. All results show mean ± standard deviation of n = 5. Each analysis was performed using averages of 5 different randomized regions on the fluorescent images of Picrosirius red staining for each sample. LdECM: Lung-derived ECM Hydrogels, Ru-LdECM: Ruthenium-crosslinked Lungderived ECM Hydrogels. Applied statistical test: paired-t test to compare the LdECM and RuLdECM hydrogels that were generated in the same experimental batch. P < 0.05 was considered statistically significant. Fibre Length Average Curvature (Δ°) P values LdECM Ru-LdECM 10 μm fibres 42.40 ± 0.92 44.98 ± 0.89 0.019 20 μm fibres 53.47 ± 0.98 56.02 ± 0.92 0.022 30 μm fibres 59.12 ± 0.71 60.84 ± 0.86 0.027 40 μm fibres 62.55 ± 0.51 63.29 ± 1.02 0.12 50 μm fibres 64.32 ± 0.79 64.60 ± 0.84 0.58
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