222 Chapter 9 Figure 1: Deposited collagen amounts in empty and fibroblast-seeded lung ECM-derived hydrogels. Control or IPF primary lung fibroblasts were seeded in control or IPF lung ECM-derived hydrogels and cultured for 7 or 14 days. Collagens were visualized using PicroSirius Red (PSR) staining on sections of paraffin-embedded fibroblast-seeded hydrogels and staining levels were compared with their corresponding empty hydrogel samples. A) Example images for the three levels of the strength of PSR staining. Day 7 responses with respect to B) weak C) moderate and D) strong PSR-stained areas (% area). Day 14 responses with respect to E) weak F) moderate and G) strong PSR-stained areas (% area). The dotted line shows the intrinsic difference between the IPF and control empty hydrogels. The estimate (± 95% confidence interval) shows the difference in PSR-staining between the IPF and control hydrogels seeded with control (light blue, triangle) or IPF (dark blue, square) fibroblasts. P values below each fibroblast group represent the differences induced by fibroblasts in IPF versus control hydrogels compared to the intrinsic difference between empty IPF and control hydrogels. P-values above the estimates indicate the differences between the responses of IPF and control fibroblasts in the different hydrogels. Applied statistical test: mixed-model analysis. ns: not significant, IPF: Idiopathic pulmonary fibrosis. n=6 for fibroblast donors, 1 image per sample was analyzed.
RkJQdWJsaXNoZXIy MTk4NDMw