244 Chapter 9 Supplementary Figure 2: Deposited glycosaminoglycan amounts in empty and fibroblast-seeded lung ECM-derived hydrogels. Control or IPF primary lung fibroblasts were seeded in control or IPF lung ECM-derived hydrogels and cultured for 7 or 14 days. Glycosaminoglycans were visualized using Alcian Blue (AB) staining on sections of paraffin-embedded fibroblast-seeded hydrogels and staining levels were compared with their corresponding empty hydrogel samples. Day 7 responses with respect to A) weak B) moderate and C) strong AB-stained areas (% area). Day 14 responses with respect to D) weak E) moderate and F) strong AB-stained areas (% area). The dotted line shows the intrinsic difference between the empty IPF and control hydrogels. The estimate (± 95% confidence interval) shows the difference in AB-staining between the IPF and control hydrogels seeded with control (light blue, triangle) or IPF (dark blue, square) fibroblasts. P values below each fibroblast group represent the differences induced by fibroblasts in IPF versus control hydrogels compared to the intrinsic difference between empty IPF and control hydrogels. P-values above the estimates indicate the differences between the responses of IPF and control fibroblasts in the different hydrogels. Applied statistical test: mixed-model analysis. ns: not significant, IPF: Idiopathic pulmonary fibrosis. n=6 for fibroblast donors, 1 image per sample was analyzed.
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