281 Fibroblast remodeling of extracellular matrix is directed by the fibrotic nature of the threedimensional microenvironment Supplementary Figure 39: Image analysis strategy used to analyze images of PicroSirius Red stained-slides. 8-bit images were converted to grayscale images and each pixel was assigned a strength between 0-255. Based on the strength values, the gel background was detected between 20 - 55 pixel strengths, and the remaining values (55 - 255) were divided into three categories to compare: weak staining (left), moderate staining (middle) and strong staining (right). 9
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