282 Chapter 9 Supplementary Document 1: ImageJ Macro used to analyze the images of PicroSirius Red-stained slides name=getTitle(); print(name); display=getTitle(); run(“Set Measurements...”, “area mean modal integrated median skewness kurtosis area_ fraction limit display redirect=None decimal=6”); run(“Colour Deconvolution”, “vectors=[ Picro_Sirius_Red]”); // Vector Picro_Sirius_Red = // Picro_Sirius_Red, 0.650,0.700,0.450, 0.150,0.750,0.400, 0.200,0.500,0.800 selectWindow(name+”-(Colour_1)”); setAutoThreshold(“Default”); setThreshold(195, 200); run(“Measure”); setThreshold(156, 194); run(“Measure”); setThreshold(117, 155); run(“Measure”); setThreshold(78, 116); run(“Measure”); setThreshold(39, 77); run(“Measure”); setThreshold(0, 38); run(“Measure”); run(“Close All”);
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