METABOLIC PROFILE CHANGES IN SERUM OF MIGRAINE PATIENTS DETECTED USING 1H-NMR SPECTROSCOPY 35 2 Results Study population We conducted a case-control study with in the ERF population cohort and included 2,088 participants in the study of which 360 were lifetime migraine patients and 1,728 without severe headache served as controls. Eight-hour fasting serum samples were available from 313 migraine patients and 1,512 controls that were used for 1H-NMR spectroscopy profiling (see Figure 1). Figure 1 Flow chart of the patient flow and analysis steps Signal detection, assignment and processing A total of 100 metabolite signals were detected in the JRES projection and quantified in the 1H-NMR spectra.For 82 signals,metabolites could be assigned.These 82 signals represented 49 different metabolites (See Additional file 1 for signal assignment).The other 18 signals could not be annotated. In total, goodquality 1H-NMR spectra were obtained from 289 migraine patients and 1,360 controls. For 19 signals (out of 100) outliers were removed and nine signals were log10-transformed (See Additional file 1). The remaining data points of the 100 signals were used for the association analyses with migraine.
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