153 Local Immune Response in Burn Patients Figure 1. High number of immune cells infiltrate the skin as response to burn injury. (A) CD45 immunohistochemical DAB staining of a representative section of healthy skin and burn tissue (from 15 days post burn) (black scale bar = 100 μm). Flow cytometry-based quantification of: (B) Absolute number of leukocytes per mg tissue (based on side scatter and CD45); (C) Proportion of granulocytes (Gran), monocytic cells (Mon) and lymphocytes (Lym) in tissue (based on side scatter and CD45); (D) Absolute numbers of granulocytes, monocytic cells and lymphocytes per mg tissue (based on side scatter and CD45). Microscopic image of multiplex DAPI, CD15 (granulocytes) and CD3 (T cells) staining of a representative: (E) Healthy skin sample; (F) Burn tissue sample (from 25 days post burn), shown separately and as composite (black scale bar = 100 μm). P values were calculated using Mann-Whitney U statistical test, significant differences are indicated by black asterisks: *p < 0.05; **p < 0.01; ***p < 0.001. 5
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