174 Chapter 5 Supplementary Figure 2. Cell counts in burn tissue. Flow cytometry-based quantification of: (A) Percentage of leukocytes that stained negative for 7-AAD or propidium iodide (viable cells); (B) Percentage of isolated cells that is CD45¯ (fibroblasts, keratinocytes, endothelial cells, others); (C) Number of eosinophils (CD9+CD16¯ granulocytes) per mg tissue; (D) Percentage of macrophages that is positive for CD40, CD80, CD163 or CD206; (E) Percentage of T cells that are CD4¯ and CD4+. (F) Number of γδ T cells (γδTCR+) per mg tissue; (G) Percentage of NK cells (CD56+ lymphocytes) within leukocyte population; (H) Percentage of B cells (CD19+ lymphocytes) within leukocyte population. P values were calculated using Mann-Whitney U statistical test, significant differences are indicated by black asterisks: *p < 0.05; **p < 0.01; ***p < 0.001.
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