184 Chapter 6 FSEs, cytokeratin 10 was expressed in all suprabasal layers of the epidermis. While in MatriDerm and Mucomaix-based FSEs, the presence of cytokeratin-10-positive cells at T0 was limited, the expression of cytokeratin 10 at T + 2 weeks was consistent in the suprabasal layer. Late differentiation marker involucrin was expressed in the stratum granulosum in ex vivo human skin from T0 onward. The DED-based FSEs showed suprabasal involucrin expression at T0, which shifted to the stratum granulosum at T + 2 weeks. As for the MatriDerm- and Mucomaix-based FSEs, involucrin was present in all suprabasal layers from T0 onward. Expression of stress marker cytokeratin 17 was not present at T0 in the ex vivo human skin models (Supplementary Figure 1C). However, when the ex vivo human skin models were cultured for 1 or 2 weeks, cytokeratin 17 expression was upregulated, similar to the FSEs. In FSEs, cytokeratin 17 was displayed in all epidermal layers. Fibroblast distribution was visualized by analyzing the presence of vimentin in cells in the dermal part of the FSEs (Figure 2D). All FSE models showed a fibroblast-populated dermis with a balanced distribution throughout the matrices. Expression of α smooth muscle actin was studied, but it was not detected in any of the FSEs.
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