188 Chapter 6 Figure 4. Evaluation of proliferation and re-epithelization in burn-injured skin models. Images of ex vivo human skin (left) and full skin equivalents generated from de-epidermalized dermis, MatriDerm and Mucomaix (right) at T0 and T + 2 weeks after burn. Immunohistochemical (A) Ki67; (B) BrdU DAB staining. Because the culture of ex vivo human skin models started at T0 and BrdU needed to be added 24 hour prior to termination of the models, no BrdU was present in these models at T0. (C) Length of re-epithelization after burn injury at T + 1 week and T + 2 weeks after burn (diamonds represent the mean per model and squares represent the mean of all models). Models were produced from 3 different skin donors in duplicate. For the full skin equivalent models, T0 was after the initial 3 weeks of culture. Black scale bar = 100 µm. Statistically significant differences are indicated by asterisks: *: p < 0.05.
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