189 Full Skin Equivalent Model for Burn Wound Healing Cytokine response of burn-injured full skin equivalent models Cytokine response in the FSEs was explored by determining the cytokine levels in the culture medium at T0, T + 1-4 days, T + 5-7 days, and T + 8-11 days (Figure 5 and Supplementary Figure 3). Of the 13 cytokines that were analyzed, high levels of IL-6, IL-8, and MCP-1 and moderate levels of IL-4 and IP-10 were found in both burn-injured and uninjured models. Only low levels of IL-12p70 and IFN-γ were detected in both burn-injured and uninjured models, while the levels of IL-2, IL-17A, and TNF-α were undetectable. The expression of IL-1β was the highest in ex vivo human skin models, and IL-10 expression appeared to be higher in both ex vivo human skin models and Mucomaixbased FSEs. TGF-β1, on the other hand, was more abundantly expressed in the FSEs than in the ex vivo human skin model. In this explorative analysis, it seemed that the expression of IL-4, IL-6, IL-8, MCP-1, IFN-γ, and IL-12p70 was more or less consistent over time, as opposed to IL-1β, IL-10, IP-10, and TGF-β1. The levels IL-1β, IL-10, and IP-10 gradually decreased over time in the ex vivo human skin models, but this was not significant. In response to burn injury, the level of IL-4, IL-6, IL-10, and TGF-β1 showed a modest increase only in MatriDerm and Mucomaix-based models at T + 1-4 days, although it did not reach significance. In the Mucomaix-based FSE, burn injury also increased the expression of IL-8, IL-12p70, and IFN-γ. Surprisingly, no differences were found for the ex vivo human skin model or DEDbased FSE in reaction to the burn injury. Thus, high levels of pro-inflammatory cytokines were present in the medium of FSE models, similar to ex vivo human skin. The effect of burn injury on pro-inflammatory cytokines was limited and was only evident in the MatriDerm and Mucomaix-based FSEs. 6
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