201 Full Skin Equivalent Model for Burn Wound Healing SUPPLEMENTARY MATERIAL Supplementary material is linked to the online version of the article at: https://www. mdpi.com/2079-4983/14/1/29. Supplementary Table 1. Culture medium for cells and full skin equivalents. Medium Contents Culture medium Dulbecco’s Modified Eagle’s Medium (DMEM), 10% fetal calf serum (Fetalclone III, Logan, UT); 1% 200 mM glutamine, antibiotics (100 IU/mL penicillin, 100 mg/mL streptomycin; Invitrogen) FSE I medium DMEM/Ham’s F12 Nutmix (3:1) (Invitrogen), 5% fetal calf serum (Fetalclone III, Logan, UT), 1 mM hydrocortisone, 1 mM isoproterenol, 0.1 mM insulin, a lipid supplement (25 mM palmitic acid, 15 mM linoleic acid, 7 mM arachidonic acid, and 24 mM bovine serum albumin) (all Sigma-Aldrich), antibiotics (100 IU/mL penicillin, 100 mg/mL streptomycin; Invitrogen) FSE II medium DMEM/Ham’s F12 Nutmix (3:1) (Invitrogen), 2% fetal calf serum (Fetalclone III, Logan, UT), 1 mM hydrocortisone, 1 mM isoproterenol, 0.1 mM insulin, 1.9 μM DL-a-tocoferol, a lipid supplement (25 mM palmitic acid, 15 mM linoleic acid, 7 mM arachidonic acid, and 24 mM bovine serum albumin) (all Sigma-Aldrich), antibiotics (100 IU/mL penicillin, 100 mg/ mL streptomycin; Invitrogen) FSE III medium DMEM/Ham’s F12 Nutmix (3:1) (Invitrogen), 0.5% fetal calf serum (Fetalclone III, Logan, UT), 1 mM hydrocortisone, 1 mM isoproterenol, 0.1 mM insulin, 1.9 μM DL-a-tocoferol, 130 mg/mL ascorbic acid, a lipid supplement (25 mM palmitic acid, 15 mM linoleic acid, 7 mM arachidonic acid, and 24 mM bovine serum albumin) (all Sigma-Aldrich), antibiotics (100 IU/mL penicillin, 100 mg/mL streptomycin; Invitrogen) 6
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