229 Immune Cell Model for Burn Immune Reactions SUPPLEMENTARY MATERIAL Supplementary Table 1. Culture media used for cell and FSE culture. Medium Contents Culture medium Dulbecco’s Modified Eagle’s Medium (DMEM), 10% fetal calf serum (Fetalclone III, Logan, UT); 1% 200 mM glutamine, antibiotics (100 IU/mL penicillin, 100 µg/mL streptomycin (all Invitrogen)) FSE I medium DMEM + Ham’s F12 Nutmix (3:1) (Invitrogen, Paisley, UK), 5% fetal calf serum (Fetalclone III), 1.1 µM hydrocortisone, 1 µM isoproterenol, 0.09 µM insulin, a lipid supplement (25 µM palmitic acid, 15 µM linoleic acid, 7 µM arachidonic acid, and 24 µM bovine serum albumin (all Sigma-Aldrich)), antibiotics (100 IU/mL penicillin, 100 µg/mL streptomycin) FSE II medium DMEM + Ham’s F12 Nutmix (3:1) (Invitrogen), 2% fetal calf serum (Fetalclone III), 1.1 µM hydrocortisone, 1 µM isoproterenol, 0.09 µM insulin, 1.9 μM DL-α-tocoferol, 5.01 µM β-cyclodextrin, 10.1 µM L-carnitine, 9.99 µM serine, a lipid supplement (25 µM palmitic acid, 15 µM linoleic acid, 7 µM arachidonic acid, and 24 µM bovine serum albumin), antibiotics (100 IU/mL penicillin, 100 µg/mL streptomycin) FSE III medium DMEM + Ham’s F12 Nutmix (3:1) (Invitrogen), 0.5% fetal calf serum (Fetalclone III), 1.1 µM hydrocortisone, 1 µM isoproterenol, 0.09 µM insulin, 1.9 μM DL-α-tocoferol, 5.01 µM β-cyclodextrin, 130 µg/mL ascorbic acid, 10.1 µM L-carnitine, 9.99 µM serine, a lipid supplement (25 µM palmitic acid, 15 µM linoleic acid, 7 µM arachidonic acid, and 24 µM bovine serum albumin (all Sigma-Aldrich)), antibiotics (100 IU/mL penicillin, 100 µg/ mL streptomycin) 7
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