Patrick Mulder

230 Chapter 7 Supplementary Table 2. Antibodies used for flow cytometry. Panel Primary antibody Clone Conjugate Macrophage anti-CD11b REA713 FITC Miltenyi Biotec GmbH anti-CD14 REA599 VioBlue anti-CD16 REA423 APC anti-CD68 REA886 APC-Vio 770 anti-CD163 REA812 PE anti-HLA-DR L243 PerCP/Cyanine5.5 BioLegend T cell anti-CD3 REA613 APC-Vio770 Miltenyi Biotec GmbH anti-CD4 REA623 VioBlue anti-CD25 REA945 PE-Vio770 anti-CD127 REA614 VioBright FITC anti-CD183 (CXCR3) G025H7 Briljant Violet 510 BioLegend anti-CD194 (CCR4) REA279 PE Miltenyi Biotec GmbH anti-CD196 (CCR6) REA190 APC Supplementary Table 3. Antibodies used for immunohistochemistry. Primary antibody Clone Host Dilution Manufacturer Antigen retrieval anti-CD3 Sp7 Rabbit 1/200 Abcam EDTA anti-CD14 Sp192 1/150 Sigma-Aldrich Citrate anti-CD68 KP1 Mouse 1/2000 DAKO EDTA BrdU IIB5 1/200 MP Biomedicals HCl/Borax

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