278 Appendix DATA MANAGEMENT PLAN Data was collected and stored following FAIR principles to increase findability, accessibility, interoperability and reuse of the datasets. Primary and secondary data obtained during this PhD project were collected and stored in the electronic labjournal (OneNote, Microsoft) and at the local server of the Association of Dutch Burn Centres in Beverwijk. The labjournal contains an overview of all performed experiments, including research questions, discussion, conclusions, succeeding steps and references to the location of raw data. Servers were well-secured by and backed up every day by the Information Technology Department of the Dutch Burns Foundation. All data archives on the server are accessible by the associated staff members. Data will be stored for at least 15 years after finalization of this project (January 31st, 2023). Part of the optimization experiments for flow cytometry of single cells isolated from burn wound and skin tissue (described in Chapter 5) were performed at the Laboratory of Medical Immunology, Department of Laboratory Medicine, Radboudumc. These data were stored at both the server of the Association of Dutch Burn Centres and the local server of the Radboudumc. Published data generated or analyzed in this thesis are part of published articles and its additional files are available from the associated corresponding authors on request. The collection and analysis of blood samples from burn patients and healthy volunteers, described in Chapter 4, were conducted in accordance with the principles of the Declaration of Helsinki. The study protocol with numbers “NL54823.094.15” (for patient samples) and “NL54823.094.15” (for volunteer samples) was approved by the METc of the VU Medical Center (Amsterdam, the Netherlands). Burn wound tissue was obtained from patients who underwent eschar debridement as part of their treatment at the Burn Center of the Red Cross Hospital in Beverwijk, the Netherlands. Healthy skin samples were used from abdominal, leg or arm reconstructions wherein excess skin was removed were obtained from adult patients who underwent elective surgery (excision of excess abdominal leg or arm skin) at the Department of Plastic and Reconstructive Surgery of the Red Cross Hospital. Consent for the use of these anonymized, post-operative residual tissue samples was received through the informed opt-out protocol of the Red Cross Hospital, which was in accordance with the national guidelines (https://www.coreon.org/) and approved by the institutional privacy officers. Subjects were actively informed of this procedure and were able to easily withdraw at any point. The privacy of the participants was secured by use of encrypted and unique individual subject codes.
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