42 Chapter 2 months (rat), >6 months (pig), >12 weeks (hamster), >12 months (rabbit), >6 months (Guinea-pig), >1 year (dog) of age. For wound depth the following categories were used: superficial (“first degree”), partial-thickness (“second degree”), deep dermal (“deep second degree”) and full-thickness (“third degree”, “fourth degree”, “severe burn injury”). P values were based on the CI95% of the difference between subgroups. For both longitudinal and subgroup analyses, Bonferroni correction was applied, i.e., the p values were multiplied by the number of comparisons within each subgroup analysis. Differences between baseline controlled studies and studies that used a separate control group were assessed. Meta-regression Meta-regression analyses were performed posthoc on the standardized mean difference of cell counts and cell function using time after burn injury as a continuous variable, including PBD 0 until PBD 21 (Supplementary File 2). Random effects‒restricted maximum likelihood model was used, and repeated measures (same animal, multiple sampling times) of studies were included. Studies included in meta-analysis [29,48,57–236] Studies included in systematic review [29,48,57–302] Baseline-controlled studies that were used for risk of bias assessment [48,65,71,73,79,80,85,86,98,113,114,134,146,157,158,160,180,187– 189,190,201,210,220,224,227,241,247–249, 254,255,260–262,264,272,280,295,303] Studies with uninjured controls that were used for risk of bias assessment [61,63,66,67,74,92,103,104,109,122,131,133,136,149,175,177,186,204,205,236,239,251, 252,257,304] ACKNOWLEDGMENTS We want to thank Alice Tillema of the Radboud University Medical Center Library for helping to design the search strategy; Carlijn Hooijmans of SYstematic Review Centre for Laboratory animal Experimentation for her assistance with the data analysis; and Anouk Elgersma, Rosa Rentenaar, and Myrthe Witbaard of the Association of Dutch Burn Centres for their assistance during data extraction. This research was funded by the ZonMw More
RkJQdWJsaXNoZXIy MTk4NDMw