71 Review Inflammatory Mediators in Animal Burn Models Cytokine response levels are associated with burn size and burn agent Next, we investigated differences in inflammatory mediator levels caused by type and severity of burn injury through subgroup analyses on percentage of TBSA, wound depth, injury site and burn agent (Figure 6). Burn size was positively associated with blood levels for IL-1β, IL-6, IL-10 and TNF-α and wound levels of IL-6 in case of >25% burns as compared to 5-25% burns. In post-hoc meta-regression analysis, blood IL-6 and IL-10 positively correlated with the reported percentage of TBSA with a p value of 0.0452 and 0.0231, respectively. Surprisingly, blood TNF-α and wound IL-6 levels were higher in burns affecting an area of ≤5% as compared to burns affecting a larger area (5-25%). Wound depth was less significantly associated with cytokine responses than burn size. In full-thickness burns only the level of TNF-α in wounds was higher compared to partialthickness burns. Blood IL-6 levels were higher in animals that were burned on both sides as compared to animals with only dorsal burns, which was likely related to the difference in burn size. The type of burn agent affected the level of inflammatory mediators in blood, but not in burn wound tissue. Blood IL-1β, IL-6 and IFN-γ levels were higher in burns caused by hot water as compared to burns created by contact with hot objects. 3
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