107 Limited genetic diversity of blaCMY-2-containing IncI1-pST12 plasmids from Enterobacteriaceae Table 4. Shufflon segments in variable regions of the different plasmids included (direction: ′5–′3). Plasmid Shufflon Segments pEC1 PilV A B rci pEC2 PilV A B rci pEC3 PilV A B rci pEC4 PilV B rci pEC5 PilV B rci pEC6 PilV A B rci pEC7 PilV B rci pEC8 PilV A B rci pEC9 PilV A B rci pEC10 PilV A B rci pEC11 PilV B A rci pEC12 PilV B rci pEC13 PilV B rci pSE1 PilV B rci Discussion The current study included E. coli isolates of various sequence types and a S. enterica isolate, which were from both human and broiler origin. Plasmid analysis based on short- and long-read sequence data of blaCMY-2 containing IncI1-pST12 plasmids from the included isolates revealed a low number of SNP differences and a high number of shared genes between the various plasmids extracted. Despite the tendency of median SNP increase from epidemiologically related to unrelated plasmids, the range in number of SNPs detected overlapped between every classified epidemiological link in the current study. A small SNP difference was seen between epidemiologically related strains with 6
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